FastQCFastQC Report
Thu 26 Sep 2019
HFJWNBGXC_n01_0813-7RG-ABF3-Endo.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFJWNBGXC_n01_0813-7RG-ABF3-Endo.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17359399
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT1280540.7376637866322446No Hit
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGA637620.3673053427713713No Hit
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGT399560.2301692587398907No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGA396920.22864846876323308No Hit
TTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAG269390.1551839438681028No Hit
CTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGAC218110.12564375068514758No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTACT262100.048.8106351
TGTACTC280200.045.908392
TACTCTA281750.045.5695274
GTACTCT286750.044.750273
ACTCTAG307900.044.5879025
CTCTAGT294850.043.47486
TCTAGTT304550.042.0870867
CTAGTTG333350.038.0941628
TAGTTGT354700.035.8990639
GTTACCT367650.034.28103314
ACCTCTA370650.033.88995717
CTAATGC372100.033.4947221
TGTTACC376350.033.46996313
ACCTACT283850.033.3289233
ACACCTA284350.033.24579231
TACCTCT380400.033.0489316
TTACCTC383000.032.98002215
GGACACC373000.032.60731529
GACACCT375500.032.50207530
CCTCTAA386800.032.49314518
Produced by FastQC (version 0.11.5)