FastQCFastQC Report
Thu 26 Sep 2019
HFJWNBGXC_n01_0813-7R-ABF3-Rest.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFJWNBGXC_n01_0813-7R-ABF3-Rest.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20413379
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT580580.28441151266529663No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG320670.1570881528236947No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC313320.1534875730274738No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG281910.13810060549015427No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT281790.13804182051388944No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA273500.13398075840359405No Hit
GCCAAATGTTTGAACGATCGGGAATTGGATCCGGTACCGAGCTCGAATTC251210.12306144906240168No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGA232980.11413103141816944No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAAC205190.10051741066483898No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG201050.037.031515
GCTGACT126600.035.250331
GACTCTA144000.031.2835944
CTAGCAG143900.031.13438
TACTCTA103800.029.674674
CTGACTC153450.029.151172
CTAGTTG104800.028.756228
TCTAGTT111050.028.0832217
CTCTAGT114100.027.1808136
GTACTCT113000.027.1036663
CTCTAGC171650.026.5306516
TGTACTC114350.026.5078052
TTGTACT115450.026.0426981
GGACACC112450.025.39817629
TGACTCT180650.025.0917073
ACACCTA87500.025.07991231
ACCTCTA118050.024.9340217
GACACCT114350.024.91489230
ACCTACT89300.024.7701133
CTAATGC119600.024.61111821