FastQCFastQC Report
Thu 26 Sep 2019
HFJWNBGXC_n01_0813-6RG-EV-Endo.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFJWNBGXC_n01_0813-6RG-EV-Endo.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29450286
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT619540.2103680758821833No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT351700.11942159067657272No Hit
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGA300040.10188016510264111No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG266550.034.169735
TACTCTA189150.033.7540254
GTACTCT205700.030.9700553
CTAGTTG205700.030.9175878
TGTACTC207950.030.516982
TTGTACT207000.030.4025651
CTCTAGT212650.030.2549236
TCTAGTT214450.029.9988387
GGACACC214000.027.918629
GTTACCT228700.027.28641914
ACACCTA168700.027.17842131
CTAATGC227600.027.17243821
GACACCT223100.026.8738730
ACCTACT170800.026.7415333
ACCTCTA232400.026.73147217
TAGTTGT250350.025.5006459
TACCTCT249550.024.8523116
ATGCTGG246050.024.85080724
TGTTACC252500.024.82536113
TGGACAC244800.024.70625728
Produced by FastQC (version 0.11.5)