FastQCFastQC Report
Thu 26 Sep 2019
HFJWNBGXC_n01_0813-6R-EV-Rest.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFJWNBGXC_n01_0813-6R-EV-Rest.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21425991
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT644260.30069087586193793No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG381770.17818078986404876No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG361920.16891634090577187No Hit
GCCAAATGTTTGAACGATCGGGAATTGGATCCGGTACCGAGCTCGAATTC330370.15419123437511012No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC321540.15007007143800255No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA284290.13268464455156356No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGA266780.12451232710776365No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAAC266040.12416695218438205No Hit
AGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGA228890.1068281975848865No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT127900.039.3539161
ACTCTAG176550.034.0619285
GACTCTA162500.031.9231414
CTAGCAG159150.031.9109788
CTGACTC164450.030.7779832
CTCTAGC192300.027.0491966
TAGCAGA195350.026.104569
TGACTCT202250.025.5797143
CTATCGA205950.024.40353617
TCTATCG213100.023.66685916
GATCTAT228700.021.94542914
ATCTATC235550.021.53011715
GCAGATC238750.021.22708511
AGATCTA248400.020.4304313
AGCAGAT254250.020.16707210
TATCGAT252850.019.91860618
TAGCATG251700.019.89888227
CTAGCAT257000.019.59751126
TTCTAGC263700.019.12599824
GCATGAC263800.018.91973129
Produced by FastQC (version 0.11.5)