FastQCFastQC Report
Thu 26 Sep 2019
HFJWNBGXC_n01_0813-5R-Col-0-ABF3-RFP.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFJWNBGXC_n01_0813-5R-Col-0-ABF3-RFP.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18968465
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT544950.2872926196189307No Hit
GCCAAATGTTTGAACGATCGGGAATTGGATCCGGTACCGAGCTCGAATTC300940.1586527955741279No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG295140.1555950890069386No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG285250.15038117211909346No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC278210.1466697489754706No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA232910.12278800630414743No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGA222450.11727359066745781No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAAC214120.11288209140802906No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT116600.034.7013471
ACTCTAG144250.032.0785065
CTAGCAG132900.031.18188
GACTCTA139450.030.069684
CTGACTC143850.028.2980752
CTCTAGC161700.026.1057956
TAGCAGA168150.024.8741239
TGACTCT170200.024.4723453
CTATCGA175950.023.47218117
TCTATCG180950.022.8235416
GATCTAT196450.021.02297214
ATCTATC201900.020.57683415
GCAGATC205100.020.22208811
TAGCATG207400.019.76183127
TATCGAT212600.019.63994218
AGATCTA212000.019.59652313
CTAGCAT212850.019.23938826
GCATGAC216150.018.86464129
AGCAGAT227300.018.58589410
TTCTAGC221750.018.40412124
Produced by FastQC (version 0.11.5)