FastQCFastQC Report
Thu 26 Sep 2019
HFJWNBGXC_n01_0813-4R-Col-0-ABF2-RFP.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFJWNBGXC_n01_0813-4R-Col-0-ABF2-RFP.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19810565
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT302780.15283763991587318No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT220620.11136481973128985No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG147150.030.9471245
GCTGACT86050.026.9274371
CTAGTTG85800.025.6592928
TACTCTA87900.025.3260294
CTAGCAG96550.024.7599498
GGACACC88350.023.68999729
GCACACG35600.023.20237211
GACTCTA107350.022.5959224
GTACTCT98400.022.4099583
ACCTCTA97800.022.15202117
GACACCT94800.022.07817630
CTAATGC97700.021.99585521
ACCTACT72900.021.94085733
CTCTAGT104550.021.6614116
TCTAGTT107550.021.5441277
TGTACTC106000.020.6379952
ATGCTGG104950.020.47647524
GTTACCT107050.020.23795714
ACACCTA80550.020.11799631
AGGACTG107300.020.09289762
Produced by FastQC (version 0.11.5)