FastQCFastQC Report
Thu 26 Sep 2019
HFJWNBGXC_n01_0813-3R-Col-0-EV-RFP.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFJWNBGXC_n01_0813-3R-Col-0-EV-RFP.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27248728
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT729600.26775561780351725No Hit
GCCAAATGTTTGAACGATCGGGAATTGGATCCGGTACCGAGCTCGAATTC397390.14583800021784504No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG370120.13583019361490928No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC369410.13556963099341737No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG362980.1332098878156808No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA294900.10822523532107627No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAAC289220.106140734349141No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGA287830.10563061879438923No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT153500.036.7121851
ACTCTAG205050.031.5978055
CTAGCAG186500.031.0034778
GACTCTA194650.029.9947724
CTGACTC195700.028.8317992
CTCTAGC228000.025.7919836
TAGCAGA229750.025.4410239
CTATCGA231900.024.51029417
TGACTCT239600.024.119053
TCTATCG242250.023.59309416
GATCTAT265350.021.4337214
ATCTATC270550.020.93120615
GCAGATC274250.020.9298111
TAGCATG278250.020.35261327
CTAGCAT287350.019.81769626
AGATCTA291200.019.72330513
AGCAGAT298300.019.67664510
TATCGAT296600.019.29341518
GCATGAC290550.019.27403829
TTCTAGC294250.019.25764724
Produced by FastQC (version 0.11.5)