FastQCFastQC Report
Thu 26 Sep 2019
HFJWNBGXC_n01_0813-2RG-ABF3-Endo.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFJWNBGXC_n01_0813-2RG-ABF3-Endo.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13947661
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT1106220.7931222303151763No Hit
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGA539740.3869752785072708No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGA329010.23588901393574163No Hit
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGT285250.20451457774891432No Hit
TTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAG216020.15487901519831893No Hit
CTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGAC180170.1291757807993756No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGGG49300.052.53467640
TTGTACT230650.048.8041231
GCACACG76000.047.7109711
ACGTCTG76350.047.17018515
TGTACTC245000.046.1173672
ACTCTAG257400.045.922695
TACTCTA247050.045.8341944
GTACTCT246950.045.7110143
ACACGTC80100.045.00585613
CACACGT81450.044.4750812
CTCTAGT259050.043.5361336
CACGTCT83800.043.227414
TCTAGTT269100.041.9203577
AGCACAC88250.041.2468510
CGTCTGA92000.039.26025816
CTAGTTG290800.038.407178
CAGTCAC94450.038.16842727
AGTCACC96300.037.5078728
GAGCACA98350.037.224829
TCCAGTC97800.036.89681225
Produced by FastQC (version 0.11.5)