FastQCFastQC Report
Thu 26 Sep 2019
HFJWNBGXC_n01_0813-2R-ABF3-Rest.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFJWNBGXC_n01_0813-2R-ABF3-Rest.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35139671
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACGATATCTCGTATGC2103880.598719322101792TruSeq Adapter, Index 7 (97% over 35bp)
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT968530.2756229561739494No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT904050.2572733250689797No Hit
GCCAAATGTTTGAACGATCGGGAATTGGATCCGGTACCGAGCTCGAATTC720580.20506168085637455No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG693060.19723007651380686No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC617610.17575861766036455No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG561520.15979660139675184No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA490060.13946061134152338No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAAC456340.1298646193927086No Hit
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGA431620.12282983525941378No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGA420620.11969947015155605No Hit
CCGACATCAAGCTGGACATCACCTCCCACAACGAGGACTACACCATCGTG400700.1140306635198719No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG376060.10701864567827057No Hit
GTCTTGACCTCGGCGTCGTAGTGGCCGCCGTCCTTCAGCTTCAGCCTCAT364230.10365208029409269No Hit
CCGGCAACAGGATTCAATCTTAAGAAACTTTATTGCCAAATGTTTGAACG363280.10338173058023223No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG429100.062.2683211
ACGTCTG433850.061.64146815
CACGATA430300.060.57232334
ACACGTC442750.060.449813
GTATGCC294350.060.35566745
CGTATGC288550.060.24674244
CACACGT444850.060.14134612
TATGCCG290150.059.8059646
CACGTCT450400.059.4696914
AGCACAC450750.059.3087310
ACGATAT446600.057.9068535
CGTCTGA469400.057.01770816
GAGCACA472300.056.6918569
ATGCCGT291900.056.6656847
TATCTCG350950.056.31632239
CTCGTAT311500.056.14517242
TCGTATG304800.056.07029743
CAGTCAC478000.055.33453827
TCCAGTC482050.054.92022725
AGAGCAC489150.054.789838
Produced by FastQC (version 0.11.5)