FastQCFastQC Report
Thu 26 Sep 2019
HFJWNBGXC_n01_0813-1RG-EV-Endo.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFJWNBGXC_n01_0813-1RG-EV-Endo.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16869629
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT743790.4409047762698279No Hit
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGA312510.1852500727787197No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT265350.1572945083735985No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGA219470.13009770398625836No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC184500.10936814318797407No Hit
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGT178540.10583516685518099No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG172720.10238517989933268No Hit
GCCAAATGTTTGAACGATCGGGAATTGGATCCGGTACCGAGCTCGAATTC169900.10071353673515879No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTACT176650.043.453361
TACTCTA184750.041.6813474
TGTACTC186350.041.4170232
ACTCTAG234550.041.1744275
GTACTCT190100.040.5080683
CTCTAGT197400.039.4723636
TCTAGTT201650.038.3075567
CTAGTTG212450.035.8660358
GTTACCT228250.032.90637214
TAGTTGT235350.032.6436049
ACCTCTA231650.032.5290617
GGACACC228650.032.20648229
ACCTACT177250.031.731633
CTAATGC234550.031.63491421
ACACCTA179150.031.53219631
GACACCT233350.031.52780330
TACCTCT242300.031.08483916
TGTTACC245200.030.78874613
TTACCTC244750.030.75937515
TGGACAC241750.030.6207128