FastQCFastQC Report
Thu 26 Sep 2019
HFJWNBGXC_n01_0813-1R-EV-Rest.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFJWNBGXC_n01_0813-1R-EV-Rest.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15582953
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT592470.3802039318221649No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC368600.2365405324651881No Hit
GCCAAATGTTTGAACGATCGGGAATTGGATCCGGTACCGAGCTCGAATTC342950.22008023768023943No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG337940.21686518595031376No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG329620.21152601820720374No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA287260.18434246705358093No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAAC252560.16207454389421566No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGA230910.14818115667806994No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG214440.13761191476352397No Hit
AGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGA193830.12438592351526696No Hit
CCGACATCAAGCTGGACATCACCTCCCACAACGAGGACTACACCATCGTG192040.12323723237822766No Hit
CCTCGATCTCGAACTCGTGGCCGTTCACGGAGCCCTCCATGCGCACCTTG191620.12296770708350335No Hit
GTCTTGACCTCGGCGTCGTAGTGGCCGCCGTCCTTCAGCTTCAGCCTCAT187810.12052272762421859No Hit
CTCGAAGTTCATCACGCGCTCCCACTTGAAGCCCTCGGGGAAGGACAGCT182500.11711515782663272No Hit
CAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTGAAGCTGCG180880.11607556026126756No Hit
CCGTAATGCAGAAGAAGACCATGGGCTGGGAGGCCTCCACCGAGCGGATG171310.1099342339029066No Hit
CCGGCAACAGGATTCAATCTTAAGAAACTTTATTGCCAAATGTTTGAACG171030.10975455037309038No Hit
CCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTG169830.10898447810244953No Hit
TGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCCTCCCTG169100.10851601747114299No Hit
GTCACCTTCAGCTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCC166750.10700795927447128No Hit
CCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTGGCGGCCCTCGGC166080.1065780022566968No Hit
GTGGTCTTGACCTCGGCGTCGTAGTGGCCGCCGTCCTTCAGCTTCAGCCT162610.10435120994076028No Hit
CGCGTGATGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTC161230.10346562682952327No Hit
GTCTTCTTCTGCATTACGGGGCCGTCGGAGGGGAAGTTGGTGCCGCGCAG159700.10248378468445615No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT110050.039.7244151
ACTCTAG156200.035.966425
CTGACTC131000.033.799472
GACTCTA136900.033.2633444
CTAGCAG136850.032.3540048
CTCTAGC153750.029.5733176
TGACTCT155550.028.8925363
TAGCAGA160150.027.690319
CTATCGA173950.025.35172517
TCTATCG178650.024.70435116
TACTCTA49700.023.4520024
GCAGATC191150.023.40093811
GATCTAT191150.023.14384714
ATCTATC193850.022.82149315
CTAGTTG51600.022.587958
AGATCTA196950.022.53336713
AGCAGAT203850.022.1491510
TATCGAT205100.021.7744818
TCTAGTT58000.021.3024237
CTCTAGT57450.021.0810416
Produced by FastQC (version 0.11.5)