FastQCFastQC Report
Thu 26 Sep 2019
HFJWNBGXC_n01_0813-15R-Col-0-ABF3-RFP.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFJWNBGXC_n01_0813-15R-Col-0-ABF3-RFP.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22000940
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT516480.23475360598228984No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG368810.1676337465581016No Hit
GCCAAATGTTTGAACGATCGGGAATTGGATCCGGTACCGAGCTCGAATTC343580.15616605472311637No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG335140.15232985499710466No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC303630.13800773966930502No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA262780.11944035118499483No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAAC234190.10644545187614711No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT113900.034.2645261
ACTCTAG170350.033.472825
CTAGCAG132450.029.6763538
GACTCTA140950.028.4594154
CTGACTC139700.027.6364082
CTAGTTG73400.024.5103668
CTCTAGC166650.024.2180586
TACTCTA80500.022.915074
TGACTCT174850.022.8615113
TAGCAGA176850.022.6806439
GGACACC75550.022.5615229
CTATCGA175050.022.31326317
ACCTACT67000.021.15647133
TCTATCG182500.021.13390416
GACACCT81650.020.91864630
GTACTCT86700.020.9129413
TCTAGTT91550.020.6068767
ACACCTA69250.020.57039331
CTAATGC86250.020.49256121
ACCTCTA89950.019.57166717
Produced by FastQC (version 0.11.5)