FastQCFastQC Report
Thu 26 Sep 2019
HFJWNBGXC_n01_0813-14R-Col-0-ABF2-RFP.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFJWNBGXC_n01_0813-14R-Col-0-ABF2-RFP.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18374294
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT260210.1416163255034452No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT250980.13659300324681864No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG146950.030.9422685
TACTCTA95400.028.7288934
CTAGTTG97350.027.7925838
GTACTCT101000.027.1011983
GGACACC99150.025.91042729
ACCTACT80350.025.61264233
CTAATGC104450.025.13141421
ACCTCTA106300.025.12193317
TCTAGTT111100.025.0145027
TGTACTC109400.024.668292
GACACCT106800.024.41490630
GCTGACT74200.024.1993451
CTCTAGT115550.023.8709686
GTTACCT113200.023.74530614
TTGTACT114000.023.5494441
ACACCTA87050.023.48073831
ATGCTGG113000.023.38493724
CTAGCAG78500.022.9181148
TGGACAC114150.022.78159328
TAGTTGT123850.022.4387199
Produced by FastQC (version 0.11.5)