FastQCFastQC Report
Thu 26 Sep 2019
HFJWNBGXC_n01_0813-13R-Col-0-EV-RFP.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFJWNBGXC_n01_0813-13R-Col-0-EV-RFP.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41244975
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT977160.23691613341988932No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG513870.12458972274804385No Hit
GCCAAATGTTTGAACGATCGGGAATTGGATCCGGTACCGAGCTCGAATTC502140.12174573993559215No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC484110.11737429832361396No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG472680.11460305164447304No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA429990.10425269987434833No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT207550.036.1235771
ACTCTAG305850.030.8433255
CTAGCAG262150.029.0930188
GACTCTA272800.028.7797374
CTGACTC269700.027.9551242
CTCTAGC322950.024.1270626
TAGCAGA330300.023.3445369
CTATCGA328950.023.00311717
TCTATCG338150.022.49109816
TGACTCT348050.022.3561823
GATCTAT376200.020.09543414
ATCTATC387500.019.60875515
GCAGATC394500.019.46546211
TAGCATG388200.019.42078827
CTAGCAT401350.018.88912826
GCATGAC404300.018.55207429
AGATCTA412700.018.49627113
AGCAGAT430700.018.11387410
TTCTAGC422200.017.84015524
TATCGAT427450.017.83339318
Produced by FastQC (version 0.11.5)