FastQCFastQC Report
Thu 26 Sep 2019
HFJWNBGXC_n01_0813-12RG-ABF3-Endo.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFJWNBGXC_n01_0813-12RG-ABF3-Endo.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38759118
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT2070810.5342768635756882No Hit
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGA1075490.27748051439147814No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGA633880.16354345318177776No Hit
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGT556090.14347333703517196No Hit
TTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAG398270.10275517621427814No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTACT476750.044.306781
TACTCTA487950.043.5706834
TGTACTC497800.042.6798672
ACTCTAG546250.042.4965935
GTACTCT506350.041.9939733
CTCTAGT525150.040.5054636
TCTAGTT535150.039.62237
CTAGTTG566150.037.125088
TAGTTGT615900.034.2337959
GTTACCT615450.033.83654414
ACCTCTA624500.033.34050817
CTAATGC621850.033.21298621
GGACACC612600.033.10964229
ACACCTA472650.032.93794331
ACCTACT473050.032.85774633
GACACCT625900.032.4617130
TGTTACC647350.032.36379213
TACCTCT649200.032.1636616
TTACCTC652450.032.04107715
TGGACAC647150.031.515128
Produced by FastQC (version 0.11.5)