FastQCFastQC Report
Thu 26 Sep 2019
HFJWNBGXC_n01_0813-12R-ABF3-Rest.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFJWNBGXC_n01_0813-12R-ABF3-Rest.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33097404
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT898850.27157719076698583No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG432630.13071417927520843No Hit
GCCAAATGTTTGAACGATCGGGAATTGGATCCGGTACCGAGCTCGAATTC415230.12545696937439565No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC412490.12462910988426765No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG389910.11780682255321294No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA370640.11198461365731283No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT363600.10985755861698397No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGA354670.10715946181156687No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG297850.035.12585
GCTGACT195150.034.8136021
CTAGCAG222200.030.9367268
GACTCTA231000.029.9412694
CTGACTC234850.028.8242442
CTAGTTG143350.025.5882458
CTCTAGC276250.025.3667186
TACTCTA146400.025.2235774
TAGCAGA283000.024.4261829
TGACTCT285350.024.2750763
CTATCGA282700.024.0305417
TCTAGTT160200.023.6177657
CTCTAGT161950.023.2559366
GTACTCT160100.023.1961923
GGACACC150350.022.97647729
TCTATCG296350.022.88821616
TGTACTC164350.022.3194542
ACCTCTA161500.022.01836217
CTAATGC161750.022.00621
GACACCT157100.021.94470230
Produced by FastQC (version 0.11.5)