FastQCFastQC Report
Thu 26 Sep 2019
HFJWNBGXC_n01_0813-11RG-EV-Endo.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFJWNBGXC_n01_0813-11RG-EV-Endo.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22786981
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT1468520.644455709161297No Hit
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGA771770.3386890084298574No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGA446780.1960680969541336No Hit
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGT402350.17657012133375632No Hit
TTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAG292710.1284549278379615No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT276650.12140704378522105No Hit
CTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGAC250490.10992680425722039No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTACT324300.047.5324941
TGTACTC341750.045.2186132
TACTCTA341650.045.2015084
ACTCTAG391150.044.8600655
GTACTCT353700.043.760333
CTCTAGT354350.043.433976
TCTAGTT368500.041.715917
CTAGTTG402050.037.9041028
TAGTTGT434100.035.1778689
GTTACCT436200.034.4940714
ACCTCTA446000.033.68104617
TGTTACC454900.033.18387613
CTAATGC449300.033.13022621
TTACCTC455850.033.10689515
GGACACC441550.033.07025529
TACCTCT456450.033.0096316
ACCTACT341150.032.73753433
ACACCTA342600.032.58926431
GACACCT450500.032.553130
CCTCTAA465600.032.30830418