FastQCFastQC Report
Thu 26 Sep 2019
HFJWNBGXC_n01_0813-11R-EV-Rest.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFJWNBGXC_n01_0813-11R-EV-Rest.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33034496
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT1034100.3130364089707922No Hit
GCCAAATGTTTGAACGATCGGGAATTGGATCCGGTACCGAGCTCGAATTC550000.1664926263745631No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG549000.16618991250842755No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC535660.16215170953417904No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG499740.15127822746258943No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA472560.14305046458102463No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAAC429540.13002771405987243No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGA424460.12848992761990374No Hit
AGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGA345510.10459066788850056No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT338010.10232031389248379No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG333520.10096112863353507No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT206950.037.9201351
ACTCTAG313150.036.9429245
GACTCTA250600.032.0139964
CTAGCAG253700.031.3448758
CTGACTC252850.030.9535062
TACTCTA131200.028.2799284
CTAGTTG135250.027.6125078
CTCTAGC293700.027.4118756
TGACTCT301850.026.5783753
TAGCAGA299300.026.5340679
GGACACC138700.025.46197529
CTATCGA313400.025.08247417
TCTAGTT154150.024.9762487
CTCTAGT152300.024.9370276
TCTATCG322750.024.37749116
TGTACTC157350.023.5353152
ACCTCTA155400.023.3103617
GACACCT151000.023.29509730
GATCTAT349950.022.52288214
GTACTCT166150.022.478593
Produced by FastQC (version 0.11.5)