FastQCFastQC Report
Thu 26 Sep 2019
HFJWNBGXC_n01_0813-10R-Col-0-ABF3-RFP.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFJWNBGXC_n01_0813-10R-Col-0-ABF3-RFP.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22143930
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT610650.275764058141441No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG329450.1487766624984815No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC326810.14758446219799284No Hit
GCCAAATGTTTGAACGATCGGGAATTGGATCCGGTACCGAGCTCGAATTC319100.14410269541133847No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG315970.1426892155096227No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAAC261850.11824910934960507No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA260780.11776590695508882No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGA239530.10816959771820088No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT133450.034.5694051
ACTCTAG176450.032.315895
CTAGCAG148450.031.0992478
GACTCTA159300.029.7076154
CTGACTC157800.029.6792582
CTCTAGC181700.026.1038176
TGACTCT191600.024.5168083
TAGCAGA192000.024.2816669
CTATCGA200950.022.90327317
TCTATCG206100.022.29700516
GATCTAT226150.020.24281914
ATCTATC231450.019.79439515
GCAGATC236300.019.35906811
TATCGAT239500.019.3337518
TAGCATG241500.019.01473227
AGATCTA246400.018.6644213
CTAGCAT246650.018.5751426
AGCAGAT254800.018.32436610
TTCTAGC256000.017.95139924
GCATGAC252400.017.90232729
Produced by FastQC (version 0.11.5)