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        Note that additional data was saved in multiqc_data when this report was generated.


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        If you use plots from MultiQC in a publication or presentation, please cite:

        MultiQC: Summarize analysis results for multiple tools and samples in a single report
        Philip Ewels, Måns Magnusson, Sverker Lundin and Max Käller
        Bioinformatics (2016)
        doi: 10.1093/bioinformatics/btw354
        PMID: 27312411

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        About MultiQC

        This report was generated using MultiQC, version 1.0.dev0

        You can see a YouTube video describing how to use MultiQC reports here: https://youtu.be/qPbIlO_KWN0

        For more information about MultiQC, including other videos and extensive documentation, please visit http://multiqc.info

        You can report bugs, suggest improvements and find the source code for MultiQC on GitHub: https://github.com/ewels/MultiQC

        MultiQC is published in Bioinformatics:

        MultiQC: Summarize analysis results for multiple tools and samples in a single report
        Philip Ewels, Måns Magnusson, Sverker Lundin and Max Käller
        Bioinformatics (2016)
        doi: 10.1093/bioinformatics/btw354
        PMID: 27312411

        A modular tool to aggregate results from bioinformatics analyses across many samples into a single report.

        Report generated on 2018-11-10, 03:11 based on data in: /beegfs/mk5636/logs/html/HFJNKBGX9/merged


        General Statistics

        Showing 29/29 rows and 3/5 columns.
        Sample Name% Dups% GCM Seqs
        HFJNKBGX9_n01_NCR5_085
        39.6%
        45%
        6.0
        HFJNKBGX9_n01_NCR5_086
        39.9%
        45%
        7.3
        HFJNKBGX9_n01_NCR5_087
        41.9%
        45%
        7.2
        HFJNKBGX9_n01_NCR5_088
        40.3%
        45%
        7.1
        HFJNKBGX9_n01_NCR5_089
        58.5%
        45%
        20.5
        HFJNKBGX9_n01_NCR5_090
        52.3%
        45%
        17.1
        HFJNKBGX9_n01_NCR5_091
        55.7%
        45%
        21.1
        HFJNKBGX9_n01_NCR5_092
        49.3%
        45%
        15.5
        HFJNKBGX9_n01_NCR5_093
        49.2%
        45%
        14.2
        HFJNKBGX9_n01_NCR5_094
        45.8%
        45%
        11.3
        HFJNKBGX9_n01_NCR5_095
        51.9%
        45%
        16.8
        HFJNKBGX9_n01_NCR5_096
        52.9%
        45%
        14.5
        HFJNKBGX9_n01_NCR5_097
        57.3%
        48%
        13.2
        HFJNKBGX9_n01_NCR5_098
        61.4%
        47%
        17.3
        HFJNKBGX9_n01_NCR5_099
        66.6%
        47%
        16.8
        HFJNKBGX9_n01_NCR5_100
        65.9%
        47%
        17.5
        HFJNKBGX9_n01_NCR5_101
        64.4%
        47%
        18.1
        HFJNKBGX9_n01_NCR5_102
        70.6%
        47%
        18.5
        HFJNKBGX9_n01_NCR5_103
        64.2%
        47%
        17.5
        HFJNKBGX9_n01_NCR5_104
        68.5%
        47%
        20.0
        HFJNKBGX9_n01_NCR5_105
        54.9%
        44%
        14.8
        HFJNKBGX9_n01_NCR5_106
        50.1%
        44%
        14.1
        HFJNKBGX9_n01_NCR5_107
        50.2%
        44%
        13.2
        HFJNKBGX9_n01_NCR5_108
        49.7%
        44%
        14.9
        HFJNKBGX9_n01_NCR5_109
        49.7%
        44%
        15.5
        HFJNKBGX9_n01_NCR5_110
        51.1%
        44%
        18.5
        HFJNKBGX9_n01_NCR5_111
        49.1%
        44%
        15.2
        HFJNKBGX9_n01_NCR5_112
        53.4%
        44%
        18.1
        HFJNKBGX9_n01_undetermined
        75.3%
        44%
        13.8

        Demultiplexing Report

        Demultiplexing Report
        Total Read Count: Total number of PF (Passing Filter) reads in this library.
        Portion: The proportion of reads that represent the individual library in the entire Library Pool.

        Showing 29/29 rows and 2/2 columns.
        Sample NameTotal Read CountPortion (%)
        undetermined_library
        13,803,090
        3.2
        NCR5_085
        5,991,807
        1.4
        NCR5_086
        7,349,696
        1.7
        NCR5_087
        7,171,488
        1.6
        NCR5_088
        7,123,050
        1.6
        NCR5_089
        20,476,275
        4.7
        NCR5_090
        17,117,946
        3.9
        NCR5_091
        21,125,915
        4.8
        NCR5_092
        15,495,350
        3.6
        NCR5_093
        14,162,925
        3.3
        NCR5_094
        11,326,539
        2.6
        NCR5_095
        16,786,457
        3.9
        NCR5_096
        14,475,555
        3.3
        NCR5_097
        13,221,093
        3.0
        NCR5_098
        17,288,913
        4.0
        NCR5_099
        16,780,701
        3.9
        NCR5_100
        17,543,159
        4.0
        NCR5_101
        18,133,346
        4.2
        NCR5_102
        18,466,814
        4.2
        NCR5_103
        17,503,226
        4.0
        NCR5_104
        20,024,036
        4.6
        NCR5_105
        14,848,545
        3.4
        NCR5_106
        14,149,438
        3.2
        NCR5_107
        13,164,316
        3.0
        NCR5_108
        14,921,072
        3.4
        NCR5_109
        15,489,488
        3.6
        NCR5_110
        18,474,135
        4.2
        NCR5_111
        15,213,909
        3.5
        NCR5_112
        18,062,531
        4.1

        Run Statistics

        Run Statistics

        Showing 1/1 rows and 4/4 columns.
        Sample NameTotal # of Single-End ReadsTotal # PF Reads% Undetermined % PhiX Aligned
        4.0
        467,009,400
        435,690,815
        3.2
        2.2

        FastQC

        FastQC is a quality control tool for high throughput sequence data, written by Simon Andrews at the Babraham Institute in Cambridge.

        Sequence Quality Histograms

        The mean quality value across each base position in the read. See the FastQC help.

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        Per Sequence Quality Scores

        The number of reads with average quality scores. Shows if a subset of reads has poor quality. See the FastQC help.

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        Per Base Sequence Content

        The proportion of each base position for which each of the four normal DNA bases has been called. See the FastQC help.

        Click a heatmap row to see a line plot for that dataset.

        rollover for sample name
        Position: -
        %T: -
        %C: -
        %A: -
        %G: -

        Per Sequence GC Content

        The average GC content of reads. Normal random library typically have a roughly normal distribution of GC content. See the FastQC help.

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        Per Base N Content

        The percentage of base calls at each position for which an N was called. See the FastQC help.

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        Sequence Length Distribution

        All samples have sequences of a single length (76bp).


        Sequence Duplication Levels

        The relative level of duplication found for every sequence. See the FastQC help.

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        Overrepresented sequences

        The total amount of overrepresented sequences found in each library. See the FastQC help for further information.

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        Adapter Content

        The cumulative percentage count of the proportion of your library which has seen each of the adapter sequences at each position. See the FastQC help. Only samples with ≥ 0.1% adapter contamination are shown.

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