FastQCFastQC Report
Sat 10 Nov 2018
HFJNKBGX9_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFJNKBGX9_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13803090
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG482370.34946522843798017No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA170920.12382734590588049No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCGCA10350.019.95322868
GGTGCGC12650.016.60363467
TGCGCAT12550.016.45521269
AGGTGCG12950.015.94867766
CTAGCAG13150.014.8985868
CGCGGGG3303.103196E-813.8920551
ACTCTAG16650.013.4492745
CTGACTC15300.012.35917952
TCCCTAC7200.012.1475949
CCCTACA8450.012.00671110
CCGGGGT2701.5612865E-411.7548161
GCGCGCC6650.011.58093162
GCTGACT15600.011.5287621
GTCCCCC3054.0695697E-511.47612869
CGGGAAT17350.011.3821831
TAGCATG16100.011.3056727
GGGGCAC2200.00536503811.2205061
TCTATCG15350.011.16827816
CTAGCAT16050.011.12263526
TCTAGCA25250.010.807277
Produced by FastQC (version 0.11.5)