FastQCFastQC Report
Sat 10 Nov 2018
HFJNKBGX9_n01_NCR5_112.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFJNKBGX9_n01_NCR5_112.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18062531
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT258040.14285927038685775No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGGG6250.013.4388991
CGCGTAT14700.012.856091
CGGGCAT22750.011.229851
CGGGAAT45700.011.1807031
CGGCAAT43400.011.0474831
CGGGCGC12100.010.98861515
CGGGGCC5452.1710912E-79.6322391
CGCGGAT22850.09.495941
GTGTGCG24050.09.4563288
CGGGGAT28000.09.3742321
CCGGGAT37150.09.3262881
GGCGGGC15850.09.05093213
GCCGAAT31050.08.9042631
CCGACAT47350.08.7954781
CGCGAAT17950.08.7736541
CGGGGAC18350.08.7731241
CCGGGGT14000.08.7492841
CGCTATG30050.08.73748429
CCCGGAT42700.08.6058521
TCGCGGG25250.08.59621269
Produced by FastQC (version 0.11.5)