FastQCFastQC Report
Sat 10 Nov 2018
HFJNKBGX9_n01_NCR5_111.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFJNKBGX9_n01_NCR5_111.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15213909
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGGG6350.014.88079551
CGGGGAT22200.012.2963921
CGCGTAT11200.011.5616491
CGGGCAT19000.011.4202141
CGGGCGC8100.011.23148915
CGGGAAT38500.010.7264841
CGTATGC22850.09.95550444
CCGGGAT30100.09.7667241
TATGCCG23200.09.6545946
CGGGGAG22800.09.3633461
CGGACAT24100.09.1487051
GGGGTAT16700.09.0113131
CCCGACT31800.08.8043831
GCCGGGT26000.08.6147511
CCGGCAT26350.08.5003231
CGGCAAT35750.08.4189611
CCCGCAT18400.08.3689491
CCGGGGT10051.8189894E-118.35759451
CCCGGAT32350.08.2219611
GTATGCC28950.08.22060145