FastQCFastQC Report
Sat 10 Nov 2018
HFJNKBGX9_n01_NCR5_110.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFJNKBGX9_n01_NCR5_110.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18474135
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT224060.1212830803715573No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGGG6700.014.625541
CGGGGAG25000.011.0589971
CGTATGC28400.010.9678546
CGGGAAT48900.010.5920871
CCGGGAT41550.010.4443011
GGGGAAT66100.010.3243281
CGCGGAT23400.010.0204581
CGCGAAT19450.09.8962751
CGGGCAT21300.09.6939571
CGGGGAT32300.09.6430881
TATGCCG32000.09.62514348
CGCCCTT19350.09.2239691
CGGCAAT43300.09.0522981
GCCGAAT28100.08.9671591
GTATGCC38100.08.63520647
GCCGGAT35650.08.54061
CGCGTAT13200.08.4840791
CCCGGCT24800.08.4669741
CGCGCCC6654.1996645E-78.42334662
CCGGGGT13100.08.2816921
Produced by FastQC (version 0.11.5)