FastQCFastQC Report
Sat 10 Nov 2018
HFJNKBGX9_n01_NCR5_109.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFJNKBGX9_n01_NCR5_109.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15489488
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGGG4850.017.318421
CGGGGAT24300.012.2419731
CGGGGAG20500.010.2432121
CGCGTAT11350.010.1755261
CGGGAAT39650.09.9741071
CCCGGGT16800.09.5826861
CCGGGAT29850.09.37960151
GCACACG32600.09.3376911
CGCGGAT18750.09.3327041
CGGGCCG4201.0118086E-49.1660481
CGCCCCT12900.08.9528851
CCCGACT30650.08.563861
CCCGGCT22950.08.5397291
CGCGCCT13550.08.5234111
CCCGCAT19750.08.5057551
CGGCAAT35400.08.5022511
CGGGCAT19400.08.4788091
GCGGGCC5401.9389918E-58.4253581
CGGGATT38650.08.4211651
GGGAGAT79250.08.3906011
Produced by FastQC (version 0.11.5)