FastQCFastQC Report
Sat 10 Nov 2018
HFJNKBGX9_n01_NCR5_108.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFJNKBGX9_n01_NCR5_108.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14921072
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT174060.1166538168303189No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG23450.013.43252148
CGTATGC24300.012.96209446
CGCGTAT11050.011.7185541
CGGGCCC4105.7907164E-711.0967061
CGGGAAT36250.011.0060481
GTATGCC29150.010.80588447
CGCGGAT20650.010.5076651
CGCCCAT20000.010.1492181
ACGTCTG34800.010.05460615
GCACACG32850.010.012111
CGGGGAT24850.09.9992291
CGGGCGC9100.09.99715215
CGGGCAT18950.09.7881641
ATGCCGT32200.09.7828749
CGCCCTT17150.09.5910971
CCCGACT28100.09.5900091
GCCGGAT33000.09.4386661
TCGTATG34100.09.33948745
TCGGGCG12750.09.3303317
CGCGGGG5704.1614476E-79.2098161
Produced by FastQC (version 0.11.5)