FastQCFastQC Report
Sat 10 Nov 2018
HFJNKBGX9_n01_NCR5_107.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFJNKBGX9_n01_NCR5_107.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13164316
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT147940.11237955697812176No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCGC9150.014.53144615
CGCGGGG5200.013.4604241
TCGGGCG13250.012.1471237
CGGGGAG18600.010.9130751
GGCGGGC12500.010.63693713
CGCGTAT10950.010.5470721
GCGGGCG12950.010.26739314
TAGCCCG14650.09.79530434
CGGGAAT32600.09.7691311
CGGGCAT19900.09.6725671
CGGCAAT30200.09.618411
CCGGGAT27150.09.5388051
CGCCCAT18950.09.4187461
CCCGACT26100.09.3861971
GCCGAAT21650.09.3756681
ATCGGGC16900.09.3170926
GGGGAAT44600.09.1808551
CGCCTAT8454.0017767E-119.1116721
GGCGCGC5007.312261E-69.10201468
CCGGCAT25700.08.8514081
Produced by FastQC (version 0.11.5)