FastQCFastQC Report
Sat 10 Nov 2018
HFJNKBGX9_n01_NCR5_106.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFJNKBGX9_n01_NCR5_106.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14149438
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT167990.11872556351708104No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAT10600.012.2163791
CGCGGGG5004.0745363E-1011.8994141
CGGGGAT22250.010.22421551
CGGGCAT18900.09.9995071
CGGGAAT33450.09.9397191
CGCGGAT19950.09.8240771
CGGGCCC3450.00160194939.1299851
CCGGGAT30100.09.0693211
CGGGGAG20600.09.0044111
CCGACAT32300.08.9933651
CGGCAAT30500.08.835631
CCGGGGT8951.3278623E-108.6011952
CGCGAAT13600.08.49222851
CGCCCAT19000.08.4732671
CCCGAAT24850.08.4502881
GTCGAAT38500.08.3632241
CGGGGAC15150.08.31642151
CGCGACT11450.08.2528681
GCCGGGG15800.08.1957991
CGCCTAT8151.6207196E-88.1591071
Produced by FastQC (version 0.11.5)