FastQCFastQC Report
Sat 10 Nov 2018
HFJNKBGX9_n01_NCR5_105.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFJNKBGX9_n01_NCR5_105.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14848545
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT230290.15509263702268472No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACGT22350.017.06963234
CCGTACG24250.016.45454433
GTACGTA24500.015.85708335
CGTATGC25350.014.081696546
CGCGGGG5800.013.878371
TATGCCG24850.013.66141448
GTATGCC29000.012.55116947
CGGGCGC13800.012.42439415
GCACACG32200.012.17054711
ACGTCTG35450.011.25244215
CGCGTAT11250.011.1992411
TCGTATG34000.010.91095245
GGCGGGC16500.010.81526313
TACGTAA35050.010.78426336
CGGGAAT36400.010.3839121
ATGCCGT34200.010.13150749
TCGGGCG18850.09.8378957
ACCGTAC42050.09.82229932
CGGGGAT24600.09.8164071
CCCGACT39850.09.7483981
Produced by FastQC (version 0.11.5)