FastQCFastQC Report
Sat 10 Nov 2018
HFJNKBGX9_n01_NCR5_104.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFJNKBGX9_n01_NCR5_104.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20024036
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC417380.20843949741201026No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT359910.17973898968219995No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG338180.1688870315654646No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG277270.1384685884504003No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG243330.12151895851565589No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA233300.11650997830806936No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT98750.027.1111681
ACTCTAG123850.024.0142965
GACTCTA119100.023.268014
CTAGCAG118750.023.2173948
CTGACTC127150.021.4098932
CTATCGA137650.019.54776217
TCTATCG139900.019.25838916
CTCTAGC147100.018.9104276
TAGCAGA149050.018.5445379
GTGCGCA140200.017.75431368
GATCTAT156200.017.15859214
TGACTCT162800.017.0224483
TGCGCAT146850.016.9979669
ATCTATC160650.016.87948615
TAGCATG156800.016.87859527
GGTGCGC148500.016.85750867
GCATGAC160800.016.52433229
CTAGCAT162650.016.44305626
GCAGATC173800.015.68249311
AGGTGCG159650.015.63639266
Produced by FastQC (version 0.11.5)