FastQCFastQC Report
Sat 10 Nov 2018
HFJNKBGX9_n01_NCR5_103.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFJNKBGX9_n01_NCR5_103.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17503226
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC366300.2092757072324839No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG360240.20581348832495222No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT358940.2050707680972639No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG229130.1309072967463255No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG219480.12539402736386995No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA198030.1131391436070128No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAAC187630.1071973817855063No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT82000.029.3196371
ACTCTAG105350.025.2093075
GACTCTA101700.024.1873364
CTAGCAG102550.023.4057358
CTGACTC106750.022.584412
CTCTAGC126400.019.488656
CTATCGA127100.018.55530217
TAGCAGA131700.018.4111659
GTGCGCA136150.018.41052668
TCTATCG129450.018.2996516
TGCGCAT141950.017.7814469
TGACTCT139650.017.6647723
GGTGCGC146300.017.20645567
GATCTAT144250.016.44599314
ATCTATC145650.016.36012315
TAGCATG143650.016.3521227
AGGTGCG154650.016.25479166
GCAGATC156650.015.38965411
GCATGAC154900.015.2550829
CTAGCAT155150.015.20737126
Produced by FastQC (version 0.11.5)