FastQCFastQC Report
Sat 10 Nov 2018
HFJNKBGX9_n01_NCR5_102.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFJNKBGX9_n01_NCR5_102.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18466814
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC364870.19758145611906852No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG351690.1904443289459676No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT331340.17942456126974582No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG229730.12440153455815389No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG220890.11961456914008015No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA207620.11242870589371831No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT86050.029.8114411
ACTCTAG110750.026.2858035
GACTCTA103150.025.101694
CTAGCAG105450.023.7570028
CTGACTC112950.022.6764932
CTCTAGC130550.019.7798736
CTATCGA125850.019.76821317
TCTATCG128350.019.19238916
TAGCAGA135000.019.023379
TGACTCT144800.018.3650193
GTGCGCA131900.018.15451668
GATCTAT141300.017.8291114
TAGCATG141750.017.5100427
TGCGCAT138850.017.27083669
GGTGCGC140350.017.1128267
CTAGCAT148950.016.68659426
ATCTATC149900.016.643115
GCATGAC151250.016.34128629
AGGTGCG149450.016.02392466
TTCTAGC155000.015.83128524
Produced by FastQC (version 0.11.5)