FastQCFastQC Report
Sat 10 Nov 2018
HFJNKBGX9_n01_NCR5_101.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFJNKBGX9_n01_NCR5_101.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18133346
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG427510.2357590264918565No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC381670.21047963238555092No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT379280.20916161860033994No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG242300.13362123019105243No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG238870.13172968739470367No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA228940.1262535882787435No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAAC197010.10864514469640628No Hit
CCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTG188950.10420029486008814No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT83600.034.201391
ACTCTAG107400.028.4094965
GACTCTA103950.028.2752274
CTAGCAG106100.027.338748
CTGACTC108900.026.4441282
CTCTAGC135700.022.2270156
TAGCAGA132200.021.9942389
CTATCGA134550.021.24713917
TCTATCG136600.020.95394916
TGACTCT141500.020.623663
GTGCGCA157350.019.24503568
GATCTAT153750.018.5706714
TGCGCAT164000.018.55010869
GGTGCGC164300.018.51756767
TAGCATG153550.018.48953627
ATCTATC156600.018.3446715
CTAGCAT161700.017.51345826
AGGTGCG174900.017.45529466
GCAGATC166350.017.41608211
GCATGAC164350.017.18970329
Produced by FastQC (version 0.11.5)