FastQCFastQC Report
Sat 10 Nov 2018
HFJNKBGX9_n01_NCR5_100.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFJNKBGX9_n01_NCR5_100.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17543159
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC379300.2162096347641836No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG364470.20775619715924595No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT344290.1962531377615628No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG240280.13696506997399957No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG227230.12952627289076044No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA191850.10935886746508995No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAAC179960.10258129678924986No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT80300.032.1665461
ACTCTAG106700.027.8088865
GACTCTA100150.026.1686784
CTAGCAG101950.025.2940778
CTGACTC104200.024.6484242
CTCTAGC126850.020.9641136
CTATCGA126400.020.51301617
TAGCAGA128700.020.3630039
TCTATCG132350.019.51150516
TGACTCT139500.019.1385353
GATCTAT146150.017.66870514
GTGCGCA138100.017.59283868
ATCTATC146750.017.52508515
TAGCATG145600.017.35930327
TGCGCAT144700.016.8869869
GGTGCGC144400.016.87492467
CTAGCAT152850.016.42116526
GCATGAC153300.016.41891529
AGATCTA161400.016.23773613
TTCTAGC160450.015.92629724
Produced by FastQC (version 0.11.5)