FastQCFastQC Report
Sat 10 Nov 2018
HFJNKBGX9_n01_NCR5_099.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFJNKBGX9_n01_NCR5_099.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16780701
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC355780.21201736447124586No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG347990.20737512693897592No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT334340.19924078261092906No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG235570.14038150134490804No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG227060.13531019949643341No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA182090.10851155741348349No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAAC173220.10322572340690653No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT75500.030.8258441
ACTCTAG97800.026.404245
GACTCTA94700.025.08854
CTAGCAG98950.023.6208848
CTGACTC102050.022.8362032
CTCTAGC120400.019.9657886
CTATCGA121100.019.18615217
TAGCAGA126250.019.0397329
TCTATCG124950.018.62298816
TGACTCT127500.018.4699173
GTGCGCA139550.018.11266368
TGCGCAT144900.017.54060269
GGTGCGC145750.017.46321167
GATCTAT136400.016.9053214
ATCTATC141900.016.44744115
AGGTGCG156250.016.20005666
TAGCATG141300.016.07920327
GCAGATC149300.015.72536611
CTAGCAT146250.015.55808726
AGATCTA149750.015.46805413
Produced by FastQC (version 0.11.5)