FastQCFastQC Report
Sat 10 Nov 2018
HFJNKBGX9_n01_NCR5_098.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFJNKBGX9_n01_NCR5_098.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17288913
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG407730.23583321866447013No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT366790.21215330310239863No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC329320.19048045415000933No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG228530.13218297761114303No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG218610.12644519640997673No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA215530.12466370789187267No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAAC183130.1059233741299988No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT82500.034.571951
ACTCTAG108600.028.062945
GACTCTA107300.027.3919644
CTAGCAG108350.026.5125528
CTGACTC108350.026.3844492
CTCTAGC132800.022.3168416
TAGCAGA137100.021.1059749
CTATCGA139700.020.3381217
TGACTCT145350.020.1734473
TCTATCG141050.020.14351816
GTGCGCA146700.019.32928868
TGCGCAT154000.018.54926169
GGTGCGC154050.018.52185467
GATCTAT155400.018.21560514
TAGCATG161650.017.49853327
ATCTATC161750.017.47896615
GCAGATC167250.017.46873511
AGGTGCG163900.017.40873766
CTAGCAT168750.016.84498826
AGATCTA173300.016.51584613
Produced by FastQC (version 0.11.5)