FastQCFastQC Report
Sat 10 Nov 2018
HFJNKBGX9_n01_NCR5_097.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFJNKBGX9_n01_NCR5_097.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13221093
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG263320.19916658932812892No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC217950.16485021321610852No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG186240.1408658119264421No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGA169570.1282571720810072No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT169510.12821179005396907No Hit
CCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTG133730.10114897459688091No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT55200.023.078341
ACTCTAG68800.020.801445
CTAGCAG63950.019.5328888
GACTCTA67150.019.4887524
CTGACTC69000.018.459532
CTCTAGC83050.015.6734086
TCTAGCA163250.015.6033187
GTGCGCA110000.015.43528268
TAGCAGA85900.014.8267869
CTATCGA84100.014.81201617
TGACTCT91250.014.6869833
TGCGCAT116850.014.560225569
GGTGCGC118950.014.33385167
TCTATCG89600.013.6684816
AGGTGCG125550.013.49673366
GATCTAT98800.012.53722914
ATCTATC102250.012.35389815
AGATCTA108850.011.63671513
AAGGTGC150050.011.50316965
GCAGATC111750.011.39722711
Produced by FastQC (version 0.11.5)