FastQCFastQC Report
Sat 10 Nov 2018
HFJNKBGX9_n01_NCR5_096.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFJNKBGX9_n01_NCR5_096.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14475555
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGGG4000.017.4989191
GTGTGCG20350.015.8181298
CGGGCGC20500.014.33745815
GGCGGGC21900.013.58044413
CGGCAAT40800.013.4673061
CGCGCGT14350.012.93125765
GCGGGGC4355.7480065E-1012.8727681
CGCGTAT15000.012.5992221
GCACACG30700.012.53682311
GGGGCGA17450.012.43961964
ACGTCTG31650.011.93979115
TACGTGG32850.011.93443555
GGCGCGC12050.011.91172268
TAGCCCG25850.011.64414534
GCTACGT33950.011.444015553
GTGCGAG29800.011.15417710
CACACGT34900.011.02817112
TAGGGGC18800.010.98738862
GCCAGTA31250.010.9753221
TCGGGCG31250.010.9725547
Produced by FastQC (version 0.11.5)