FastQCFastQC Report
Sat 10 Nov 2018
HFJNKBGX9_n01_NCR5_095.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFJNKBGX9_n01_NCR5_095.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16786457
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCGC17350.015.93262415
CGCGGGG5650.014.8662041
TATGCCG23800.014.26348446
CGTATGC24700.013.7436844
GGCGGGC21150.013.73163513
GTGTGCG22150.012.3215418
GCGGGCG23350.011.98844414
CGCGTAT14350.011.9503751
TAGCCCG24450.011.881824534
TCGGGCG26750.011.7723437
GCGGAAT32250.011.2860241
GGGGCGA14900.011.27843764
GGCGCGC11350.011.10377868
GTATGCC32050.011.02872945
GCACACG33900.010.94089711
CGGCAAT43250.010.84316351
CCGGGAT33350.010.598951
ATCGGGC31400.010.5864996
GGCGCAT28250.010.40463817
ATGCCGT34500.010.34712547
Produced by FastQC (version 0.11.5)