FastQCFastQC Report
Sat 10 Nov 2018
HFJNKBGX9_n01_NCR5_094.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFJNKBGX9_n01_NCR5_094.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11326539
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGGG4300.017.906211
GTGTGCG17500.016.5951888
CGGGCGC12200.015.20104815
CGCGTAT9750.013.9994021
GGCGGGC14400.013.12136913
GTGCGAG23750.012.66995410
ACCCGTA22700.012.33612632
CGGCAAT28750.011.8081911
CCGGGAT22850.011.7938061
TAGCCCG17050.011.70125934
GCGGGCG16600.011.59333114
TGTGCGA26650.011.5538739
TCGGGCG20600.011.5500497
GGCGCGC6850.011.24362468
GCACACG22450.011.2216911
CGCTATG22350.010.96455129
CGGGCAT18000.010.8884251
CCGACAT36050.010.87332251
CCCGTAA26950.010.78014133
AAGGCGC28600.010.7687738
Produced by FastQC (version 0.11.5)