FastQCFastQC Report
Sat 10 Nov 2018
HFJNKBGX9_n01_NCR5_093.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFJNKBGX9_n01_NCR5_093.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14162925
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGGG5750.015.824051
GTGTGCG15900.013.4232898
CGCGTAT13300.013.15621
GCACACG28450.011.68356111
CGGGCGC12850.011.43627715
CGGGAAT38100.011.2977571
CGGCAAT34000.010.8074321
ACGTCTG32150.010.12139815
CGCTATG28100.010.09155329
CGTATGC20850.010.07110844
TATGCCG18950.09.97264846
GGGGCGA11700.09.57574664
TCGGGCG19700.09.413177
CACACGT36100.09.40159412
GCCAGAT42600.09.3649911
CGGGCAT18450.09.2941961
GCCGGGC5653.665591E-79.2908391
ACCCGTA23500.09.23500532
GTCGCTA31200.09.20091327
CCGACAT43200.09.1539141
Produced by FastQC (version 0.11.5)