FastQCFastQC Report
Sat 10 Nov 2018
HFJNKBGX9_n01_NCR5_092.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFJNKBGX9_n01_NCR5_092.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15495350
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTGCG20150.015.454738
GGCGCGC10500.013.00290768
CGCGTAT13050.012.6047231
ACCCGTA26700.011.4052432
CGGCAAT38750.011.2897481
CGGGCGC14700.011.18766915
TAAGGCG30700.010.2603337
CCGGGAT33000.010.1813011
GTGCGAG30150.010.09664310
GGGGCGA13700.09.96650464
AGGCGCA31000.09.93491639
TAGGGGC14950.09.8352662
TGTGCGA32450.09.8123539
CGTGCGC15000.09.80158958
CCCTCGC32200.09.78555566
AAGGCGC32400.09.72194838
ACGGGTG36000.09.62301920
GTACCCC22000.09.54533948
CGGACAT27500.09.544971
CGGGGAG25000.09.5195171
Produced by FastQC (version 0.11.5)