FastQCFastQC Report
Sat 10 Nov 2018
HFJNKBGX9_n01_NCR5_091.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFJNKBGX9_n01_NCR5_091.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21125915
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGGG8600.015.8712841
GTGTGCG31600.015.6126328
GGGGCGA21550.012.18473764
GGCGCGC17650.011.90093168
GTGCGAG42550.011.84150810
CGGGCGC25650.011.59567915
ACCCGTA43900.011.48160132
TAGGGGC22150.011.22216162
TGTGCGA47350.010.8627929
CGGCAAT56600.010.8210021
CGGGCAT31850.010.7686811
CGGGGAT31000.010.4994631
GCGGAAT38200.010.4444931
CCGGGGT14500.010.378781
TGCGAGT48750.010.33570711
GGCGGGC30300.010.27795913
ATAGGGG24550.010.26754761
GCGAGTC52800.010.07323912
CGGGAAT60450.010.0160311
TCGGGCG37550.09.877347
Produced by FastQC (version 0.11.5)