FastQCFastQC Report
Sat 10 Nov 2018
HFJNKBGX9_n01_NCR5_090.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFJNKBGX9_n01_NCR5_090.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17117946
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGGG6950.019.6380081
GTGTGCG26100.014.0760698
CGGGAAT42600.012.8154131
CGTATGC23850.011.29917144
CCGGGAT35800.011.2417121
CGGGCGC19800.011.13311815
GGGGCGA16950.010.94738364
TATGCCG23550.010.84869446
TAGGGGC18850.010.77226162
CGCGTAT16150.010.6179621
CGGGGAT25400.010.6090051
GTGCGAG35300.010.60572610
ACCCGTA35800.010.55959332
GACTGTC50300.010.5039274
CCGGGGT14750.010.43946551
GCGGGCC7101.2732926E-1110.3509111
CGGGCAT24750.010.3220341
CGGCAAT43800.010.0673251
ATAGGGG20550.09.88106761
TGTGCGA38250.09.87927159
Produced by FastQC (version 0.11.5)