FastQCFastQC Report
Sat 10 Nov 2018
HFJNKBGX9_n01_NCR5_089.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFJNKBGX9_n01_NCR5_089.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20476275
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAT20250.015.555051
GTGTGCG31650.014.8144798
TATGCCG24400.014.34337646
GGCGCGC14600.013.90708868
CGGGCGC27650.013.16139715
CGTATGC28550.012.3808444
GGCGGGC31150.011.68247313
TGTGCGA42250.011.5118049
CGGGAAT56250.011.1996361
ACCCGTA40400.011.17638932
GTGCGAG42450.011.12782610
TAGCCCG33850.010.96024334
TCGGGCG40600.010.9454347
GCCGGAT42600.010.9268751
CCGGGAT42050.010.82011
CGGCAAT60000.010.7913151
ACGTCTG45000.010.73075715
TAGGGGC21550.010.72165962
CACTTAG43150.010.46507631
GCACACG44050.010.40600111
Produced by FastQC (version 0.11.5)