FastQCFastQC Report
Sat 10 Nov 2018
HFJNKBGX9_n01_NCR5_088.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFJNKBGX9_n01_NCR5_088.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7123050
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAT6950.018.1267721
GTGTGCG10300.017.6647688
CGCGGGG2405.5792694E-513.1230281
ACCCGTA13700.013.02970832
CGGGCGC6550.012.8211515
GTGCGAG14150.012.61117510
CGGCAAT18350.012.0145421
TGTGCGA16050.011.5542729
TAAGGCG15900.011.44642837
ACGGGTG16100.011.30209320
AGGCGCA15800.011.29695939
TGCGAGT15800.011.29434211
CCGGGAT14800.011.11319351
TCGCGGG15550.011.03123169
GCGGGAC5401.4624675E-911.0168621
GGCGGGC8200.010.66785413
CGGGCAT10200.010.6356551
CCCGTAA16450.010.425940533
TGGCGGG17850.010.39395257
CGGGAAT19250.010.3620781
Produced by FastQC (version 0.11.5)