FastQCFastQC Report
Sat 10 Nov 2018
HFJNKBGX9_n01_NCR5_087.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFJNKBGX9_n01_NCR5_087.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7171488
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGGG2504.1836756E-1119.59821
CGGGCGC7300.016.77670715
GTGTGCG11350.014.4889298
GGCGGGC9800.013.2108613
GTACCCC11650.012.91834948
CGCTATG15400.012.50225229
TAGCCCG11250.012.4444334
GGCGCGC5155.0931703E-1112.23623768
GCGGGCG10400.012.11241314
GTGCGAG14800.011.58426910
CGCGTAT6650.011.5778841
CGGCAAT18400.011.4119951
GTCGCTA18150.011.37938127
ACGGGTG16200.010.80027120
ACCCGTA15050.010.69833432
ACCCCGC14450.010.65772350
TCGGGCG12950.010.5372947
TAAGGCG17000.010.49984137
TCGCGGG15800.010.41392169
CGGGCAT11200.010.3115531
Produced by FastQC (version 0.11.5)