FastQCFastQC Report
Sat 10 Nov 2018
HFJNKBGX9_n01_NCR5_086.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFJNKBGX9_n01_NCR5_086.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7349696
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGGG2456.6393113E-1018.5711781
CGCGTAT6700.016.7161941
GTGTGCG9350.013.471638
GGCGCGC4558.367351E-1113.07968468
CGGGAAT22800.011.9735241
CGGGCAT9850.011.7257311
CCGGGAT16900.011.5974781
GCGGAAT13700.011.4961961
GCCGGAT15700.011.3692741
CCGGCCT9100.011.1536961
ACCCGTA12350.011.05421432
ACGTCTG14950.010.06435715
TATGCCG9400.010.05285246
CGGGCGC6008.305506E-99.91417215
CGGGGGG3955.1685718E-59.7467041
GTCGAAT23450.09.5521111
ACTCGGC14350.09.513556532
CGCGGAT12950.09.4593321
CACTCGG23100.09.39566831
GCACACG14950.09.36168411
Produced by FastQC (version 0.11.5)