FastQCFastQC Report
Sat 10 Nov 2018
HFJNKBGX9_n01_NCR5_085.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFJNKBGX9_n01_NCR5_085.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5991807
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGGG1854.0563464E-1022.7005061
CGGGCCC1704.3944783E-516.4689961
TATGCCG9150.015.29957846
GTGTGCG8700.015.2819238
ATGCCGT10200.014.06844147
CGTATGC9850.013.14593644
GCACACG12300.012.23162711
GTATGCC12100.012.14799145
ACGTCTG12400.011.85102215
CCGGGAT11100.011.6655391
ATAGGGG5451.382432E-1011.56276261
CGCGCGA3953.570549E-711.51912422
GGGGCGA5904.5474735E-1111.27470864
GTGCGAG11500.011.25696210
ACCCGTA12150.011.23629632
CACACGT13800.011.15582712
TAGGGGC5652.582965E-1011.15374162
CGGGGGG3501.363342E-510.9989361
CGCGTAT4501.6336526E-710.8878361
TCGTATG13200.010.87025243
Produced by FastQC (version 0.11.5)