FastQCFastQC Report
Thu 6 Dec 2018
HFJMNBGX9_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFJMNBGX9_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31002316
Sequences flagged as poor quality0
Sequence length99
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2701350.8713381284159544No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCAGTG399550.056.2672272
AAGCAGT543650.042.6699941
GCAGTGG544200.041.3885273
AGTGGTA570200.040.325635
CAGTGGT621350.036.4226344
GTATCAA635350.035.979839
GGTATCA646300.035.1110538
GTGGTAT666650.034.359076
TGGTATC774750.029.3134337
AGTACAT581250.023.61937520-21
ATCAACG582750.023.10487710-11
TACATGG585850.022.79736722-23
AGAGTAC614850.022.41617818-19
CATGGGG337600.022.35719924-25
CAACGCA610750.022.09483512-13
GCAGAGT647650.021.20449316-17
GTACATG549650.020.82089622-23
CGCAGAG573350.020.4118316-17
CAGAGTA606400.019.4761318-19
ACATGGG579400.019.41996424-25
Produced by FastQC (version 0.11.5)